il of CB contains α-d-glucopyranose, which can be used in medical tests and medicines.A series of high-density polyethylene nanocomposites filled with different diameter sizes (5, 15, and 25 μm) of graphene nanoplatelets at various amounts (0.5-5 wt.%) are prepared by the melt-mixing method. The effect of diameter size and filler content on the mechanical properties is reported, and the results are discussed in terms of morphology and the state of dispersion within the polymer matrix. The measured stiffness and strength of the nanocomposites were found to be mainly influenced by the filler aspect ratio and the filler-matrix adhesion. Fractography was utilized to study the embrittleness of the nanocomposites, and the observations revealed that a ductile to brittle transition is caused by a micro-deformation mechanism change in the nanocomposites. Several micromechanical models for the prediction of mechanical properties of nanocomposites, taking into consideration filler aspect ratio, percolation effect, and interphase regions, are considered. The three-phase model proposed by Ji accurately predicts the stiffness of graphene nanoplatelets with a higher diameter size, while Takayanagi modified model II was found to show good agreement with the experimental results of smaller ones at low filler content. This study demonstrates that the diameter size of the filler plays a central role in determining the mechanical properties.Hydrogels are suitable for osteochondral defect regeneration as they mimic the viscoelastic environment of cartilage. However, their biomechanical properties are not sufficient to withstand high mechanical forces. Therefore, we have prepared electrospun poly-ε-caprolactone-chitosan (PCL-chit) and poly(ethylene oxide)-chitosan (PEO-chit) nanofibers, and FTIR analysis confirmed successful blending of chitosan with other polymers. The biocompatibility of PCL-chit and PEO-chit scaffolds was tested; fibrochondrocytes and chondrocytes seeded on PCL-chit showed superior metabolic activity. The PCL-chit nanofibers were cryogenically grinded into microparticles (mean size of about 500 µm) and further modified by polyethylene glycol-biotin in order to bind the anti-CD44 antibody, a glycoprotein interacting with hyaluronic acid (PCL-chit-PEGb-antiCD44). The PCL-chit or PCL-chit-PEGb-antiCD44 microparticles were mixed with a composite gel (collagen/fibrin/platelet rich plasma) to improve its biomechanical properties. The storage modulus was higher in the composite gel with microparticles compared to fibrin. The Eloss of the composite gel and fibrin was higher than that of the composite gel with microparticles. The composite gel either with or without microparticles was further tested in vivo in a model of osteochondral defects in rabbits. PCL-chit-PEGb-antiCD44 significantly enhanced osteogenic regeneration, mainly by desmogenous ossification, but decreased chondrogenic differentiation in the defects. PCL-chit-PEGb showed a more homogeneous distribution of hyaline cartilage and enhanced hyaline cartilage differentiation.Climate change brought about by anthropogenic CO2 emissions has created a critical need for effective CO2 management solutions. Microalgae are well suited to contribute to efforts aimed at addressing this challenge, given their ability to rapidly sequester CO2 coupled with the commercial value of their biomass. Recently, microalgal biofilms have garnered significant attention over the more conventional suspended algal growth systems, since they allow for easier and cheaper biomass harvesting, among other key benefits. However, the path to cost-effectiveness and scaling up is hindered by a need for new tools and methodologies which can help evaluate, and in turn optimize, algal biofilm growth. Presented here is a novel system which facilitates the real-time in situ monitoring of algal biofilm CO2 sequestration. Utilizing a CO2-permeable membrane and a tube-within-a-tube design, the CO2 sequestration monitoring system (CSMS) was able to reliably detect slight changes in algal biofilm CO2 uptake brought about by light-dark cycling, light intensity shifts, and varying amounts of phototrophic biomass. This work presents an approach to advance our understanding of carbon flux in algal biofilms, and a base for potentially useful innovations to optimize, and eventually realize, algae biofilm-based CO2 sequestration.The paper presents a new approach to non-destructive evaluation of easy/hard magnetization axis in grain-oriented SiFe electrical steels based on the Barkhausen phenomenon and its time-frequency (TF) characteristics. Anisotropy in steels is influenced by a number of factors that formulate the global relationship and affect the Barkhausen effect. Due to the observed high variability in the dynamics of magnetic Barkhausen noise (MBN) over time, obtained for various directions in grain-oriented steel, it becomes justified to conduct MBN signal analyses in the time-frequency domain. This representation allows not only global information from MBN signal over entire period to be expressed, but also detailed relationships between properties in time and in frequency to be observed as well. This creates the opportunity to supplement the information obtained. The main aspect considered in the work is to present a procedure that allows an assessment of the resultant angular characteristics in steel. For this purpose, a sample of a conventional grain-oriented SiFe sheet was used. Measurements were made for several angular settings towards the rolling and transverse directions. A data transformation procedure based on short-time Fourier transform (STFT) as well as quantitative analysis and synthesis of information contained in the TF space was presented. Angular characteristics of selected TF parameters were shown and discussed. In addition, an analysis of the repeatability of information obtained using the proposed procedure under various measurement conditions was carried out. selleck kinase inhibitor The relationship between the selection of calculation parameters used during transformation and the repeatability of the obtained TF distributions were demonstrated. Then the selection of the final values of the calculation parameters was commented upon. Finally, the conclusions of the work carried out were discussed.